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similar purification tasks.  These commercially available de-  Conclusion
          vices vary greatly in size, weight, cost, and volume of water
          throughput. Six different methods of water purification were   Based on the results of this study and the feedback received
          evaluated and found to effectively reduce the bacterial burden   from experienced military physicians, we conclude that a
          in water. However, no single device was perfect, as each device   layered approach to water purification is optimal. Notably,
          had both perceived strengths and weaknesses.       CHLOR-FLOC, the product the U.S. Military has used since
                                                             WWII, has many desirable characteristics and effectively re-
                                                             duces microbial load in contaminated water. In austere settings
          Severe, open, battlefield wounds are inherently prone to in-
          fection because of their exposure to the environment, con-  where all equipment is manually carried, given its high-volume
          tamination with dirt and debris, and blood loss/transfusion.    throughput, low cost and cube, and positive effects on pH and
                                                         11
          Irrigation with fluids is a primary strategy to remove debris   water decontamination, the Aquamira drops appear superior
          and reduce infection.  Accordingly, it is crucial to ensure that   to the other small, lightweight products. When weight, cost,
                          12
          no additional bacteria are introduced via contamination with   and cube are less important (vehicle availability), the Guardian
          dirty water. All the evaluated products significantly decreased   purifier appears to be a superior device.
          the CFUs of all contaminated water sources (pond and river
          water and inoculated saline), but only the Steripen device re-  Author Contributions
          duced bacteria levels to below detectable limits.  NM, DJS, and JCW conceived the study. IBH and SMS co-
                                                             ordinated, collected, and analyzed the data. IBH, SMS, JBH,
                                                             and JCW wrote the first working drafts of the manuscript. All
          It is generally understood that human pathogenic bacteria
          thrive in alkaline environments; as such we measured pH in   authors read, edited, and approved the final manuscript.
                                                  13
          the pond, river, and tap waters used in this study.  The use
          of filtration or boiling decreased the already alkaline pH of   Disclaimer
          the pond, river, and tap water, whereas chemical purification   The views expressed in this article are those of the author(s)
          methods raised the acidity of the water. Accordingly, chemical   and do not reflect the official policy or position of the U.S.
          purification may be preferred to filtration.       Army Medical Department, Department of the Army, DoD, or
                                                             the U.S. Government.
          Turbidity reflects the  “cloudiness” or individual particles
          in any water source and generally indicates the presence of   Disclosures
          pathogenic microorganisms.  As expected, pre-treatment tur-  Dr. Holcomb is a consultant with BARDA, Aspen medical and
                                14
          bidity measurements indicate that only pond and river water   DoD, is Co-founder, Co-CEO and a Member of the Board of
          sources were more turbid than saline or tap water sources. The   Directors  of  Decisio  Health, the Board  of Directors  of He-
          Guardian and CHLOR-FLOC methods decreased the turbid-  mostatics, QinFlow, Zibrio, and Oxyband. He is also a Co-
          ity of both river and pond water.                  inventor of the Junctional Emergency Tourniquet Tool.

          Given the lack of conclusive data from high-quality clinical tri-  References
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