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Table 1  Descriptions of Selected ITC Training Phases  Body Mass and Energy Balance
           Training Phase,                        Food       Body mass was measured under the same experimental condi-
           No. of Days          Task            Availability  tions (i.e., after an overnight fast, morning void, and seminude)
           Survival, Evasion, Resistance, and Escape School  before and after each training phase by having all study volun-
               12      Academic instruction on   DFAC, local   teers use an electronic, calibrated scale (model PS6600; Befour,
                       survival, evasion, resistance,   economy  http://befour.com/). Total daily energy expenditure (TDEE; via
                       and escape techniques in a            doubly labeled water), energy intake (EI), and energy balance
                       classroom setting                     (EB = EI − TTDEE) were assessed in a random subset of the
                7      Simulated evasion, resistance,   Minimal a  original 20 volunteers during SERE (n = 10), RS (n = 12), CQB
                       and escape environments in            (n = 9), and DB (n = 13). Subset analyses were intended for 15
                       field settings
           Raider Spirit                                     volunteers per phase; however, the sample sizes were smaller
                                                             because of study attrition (SERE and RS), and because volun-
                9      Simulated raids and ambushes   Field rations b  teers were tasked with other duties during CQB.
                       in field settings
           Close Quarter Battle                              For TDEE assessments, volunteers provided a urine sample af-
               19      Weapon qualification and close-  DFAC, local   ter an overnight fast to determine background abundance of
                       quarter shoot house training at   economy  18  2
                       firing range                           O and  H before ingesting the doubly labeled water (0.285g
                                                               18
                                                                                                 2
           Derna Bridge                                      H O/kg total body water [TBW] and 0.15g  H O/kg TBW;
                                                                                                   2
                                                               2
                                                             Sigma-Aldrich, https://www.sigmaaldrich.com/). Volunteers
               23      Training a local guerrilla force   DFAC, local
                       and simulated irregular warfare   economy, field   remained fasted for the next 4 hours and provided urine sam-
                       operations in field settings  rations b  ples at 4 hours and 6 hours to determine TBW. Second-morn-
          This is a general overview of each training of the four selected train-  ing void urine samples were then collected daily during each
          ing phases of ITC. DFAC, dining facility; ITC, Individual Training   training  phase. Enrichments  of  H and  O  were measured
                                                                                       2
                                                                                             18
          Course; MRE, Meals, Ready-to-Eat.                  using isotope ratio mass spectroscopy (Finnigan Mat 252;
          a Participants subsisted on one ration of MRE, an uncooked chicken   Thermo Fisher Scientific, https://www.thermofisher.com). The
          and rabbit that the Marines eventually had to cook, root vegetables,   2  18
          2 cups of rice, and two pieces of bread during the 7 days of training.  H and  O isotope elimination rates were calculated by linear
          b Field rations consisted of MRE (approximately 1,300kcal/ration).  regression using the isotopic disappearance rates in the urine
                                                             samples collected during each training phase. 1,4,6,7
          into a 12-day academic phase and a 7-day field phase. The
          academic phase consists of worldwide survival, evasion, and   EI was evaluated by trained registered dietitians using 24-hour
          recovery principles; and resistance and escape techniques, in-  dietary recalls every third day in garrison. When subsisting
          cluding food and water procurement, signaling, evasion shel-  primarily on combat rations during field training, EI was as-
          ter, fire craft, survival medicine, and navigation principles.   sessed using ration-specific food logs every third day. 1,6,7  For
          These techniques are applied during the field training phase,   SERE, EI was estimated by quantifying all the food items (i.e.,
          during which students conduct evasion training, traversing   one combat ration [MRE], an uncooked chicken and a rabbit
          rough terrain while carrying an additional 20–30kg pack for   the Marines had to cook, root vegetables, 2 cups of rice, and
          up to 16 h/d. Students are then captured and exposed to sig-  two pieces of bread) provided to participants by cadre and
          nificant psychological and physiological stress during mock   averaged over the 7-day period. In addition, TDEE, EI, and
          captivity to enhance survivability skills and to develop coping   EB measurements were separated into academic and fielding
          strategies in the event of enemy capture. Food availability is   phases because SERE comprised 12 days of academic training
          highly restricted during the field phase of SERE because near-  and 7 days of field training (the academic phase of RS was too
          complete starvation is an intentional stressor uniformly ap-  short for accurate assessments of TDEE; therefore, only TDEE
          plied to all students.                             for the field phase is presented.

          RS is a 12-day exercise comprising a 3-day academic planning   Dietary Patterns and Quality
          phase and a 9-day field phase designed to prepare a CSO for   Dietary  patterns  and quality  were  assessed  at  the  start  and
          the metabolic demands and psychological stressors of a mis-  end of ITC by using the full-length, 100-item semiqualita-
          sion. During the field phase, the students are highly active, ex-  tive, 3-month version of the Block 2005 Food Frequency
          ecuting task-oriented operations, including reaction to enemy   Questionnaire (FFQ; Nutrition Quest, http://nutritionquest
          movement and to extraction while carrying a 20–30kg pack.   .com/).  These data were used to calculate the Healthy Eat-
                                                                  8,9
          Students have limited time to sleep and are provided only two   ing Index-2010 (HEI-2010), a measure of diet quality in terms
          combat rations per day (meals, ready-to-eat [MREs]; Ameri-  of  conformance  to  the  2010  Dietary  Guidelines  for  Ameri-
          qual, Evansville, IN; approximately 1,300 kcal/ration). CQB   cans. 10–12  Total HEI-2010 scores are based on 12 components
          is a 19-day weapons qualification and CBQ training exercise   that range from 0 to 100 (100 being the best).
          designed to ensure that CSOs can engage and neutralize tar-
          gets at close proximity in an enclosed space. Students spend   Statistical Analysis
          approximately 10 h/d training, have regular access to the local   Shapiro-Wilk tests of normality confirmed that all data were
          military dining facility, and also can consume food they pro-  normally distributed. Mixed-model analysis of variance, with
          cure through the local economy. DB is the ITC culmination ex-  participant as the random factor, was used to assess differ-
          ercise that lasts 23 days, during which students are expected to   ences in TDEE, EI, and EB among training phases (i.e., SERE,
          apply the skills developed over the past 9 months to complete   RS, CQB, and DB). A two-way repeated measures analysis of
          a mock mission. During DB, students rely on combat rations   variance was used to determine changes over time (i.e., be-
          (MRE), food from the local dining facility, and food items they   fore and after) and among the four training phases for body
          procure through the local economy during the training.  mass. Bonferroni adjustments were used for multiple pairwise

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